Minimal Medium

Patricia L. Clark
last updated: March 18, 2002


M9 Salts
can make as 1x, 2x, 5x, or 10x concentrate, for later addition into minimal medium (below)
final pH should be 7; autoclave for 40 min
Ingredient 1X 2X 5X 10X
Na2HPO4 5.8 g 11.6 g 29 g 58 g
KH2PO4 3.0 g 6.0 g 15.0 g 30 g
NaCl 0.5 g 1.0 g 2.5 g 5 g
NH4Cl 1.0 g 2.0 g 5.0 g 10 g
ddH20 to 1 L to 1 L to 1 L to 1 L

 

 

Minimal Medium
au = autoclave
fs = filter sterilize
Ingredient [Stock] to make 200 mL to make 500 mL to make 1 L
M9 salts above above above above
MgSO4 (1 mM) 1 M (au) 0.2 mL 0.5 mL 1.0 mL
FeCl3/CaCl2 (1 uM) 1 mM (au) 0.2 mL 0.5 mL 1.0 mL
Glucose (0.4%) 40% (au) 2.0 mL 5.0 mL 10 mL
His/Leu (0.0015% ea) 0.3/0.8% (fs) 1.0 mL 2.5 mL 5.0 mL
Yeast extract (0.01%) 1% (au) 2.0 mL 5.0 mL 10 mL